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SEQWORDS documentation |
TY SCOP XX CL Alpha and beta proteins (a/b) XX FO NAD(P)-binding Rossmann-fold domains XX SF NAD(P)-binding Rossmann-fold domains XX FA Lactate & malate dehydrogenases, N-terminal domain XX TE NAD(P)-binding Rossmann-fold TE Lactate & malate dehydrogenases TE Lactate dehydrogenase TE Malate dehydrogenase // |
ID ACEA_ECOLI STANDARD; PRT; 434 AA.
AC P05313;
DT 01-NOV-1988 (Rel. 09, Created)
DT 01-NOV-1988 (Rel. 09, Last sequence update)
DT 15-DEC-1998 (Rel. 37, Last annotation update)
DE ISOCITRATE LYASE (EC 4.1.3.1) (ISOCITRASE) (ISOCITRATASE) (ICL).
GN ACEA OR ICL.
OS Escherichia coli.
OC Bacteria; Proteobacteria; gamma subdivision; Enterobacteriaceae;
OC Escherichia.
RN [1]
RP SEQUENCE FROM N.A.
RC STRAIN=K12;
RX MEDLINE; 89083515.
RA Byrne C.R., Stokes H.W., Ward K.A.;
RT "Nucleotide sequence of the aceB gene encoding malate synthase A in
RT Escherichia coli.";
RL Nucleic Acids Res. 16:10924-10924(1988).
RN [2]
RP SEQUENCE FROM N.A.
RC STRAIN=K12;
RX MEDLINE; 88262573.
RA Rieul C., Bleicher F., Duclos B., Cortay J.-C., Cozzone A.J.;
RT "Nucleotide sequence of the aceA gene coding for isocitrate lyase in
RT Escherichia coli.";
RL Nucleic Acids Res. 16:5689-5689(1988).
RN [3]
RP SEQUENCE FROM N.A.
RX MEDLINE; 89008064.
RA Matsuoka M., McFadden B.A.;
RT "Isolation, hyperexpression, and sequencing of the aceA gene encoding
RT isocitrate lyase in Escherichia coli.";
RL J. Bacteriol. 170:4528-4536(1988).
RN [4]
RP SEQUENCE FROM N.A.
RC STRAIN=K12 / MG1655;
RX MEDLINE; 94089392.
RA Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J.,
RA Daniels D.L.;
RT "Analysis of the Escherichia coli genome. IV. DNA sequence of the
RT region from 89.2 to 92.8 minutes.";
RL Nucleic Acids Res. 21:5408-5417(1993).
RN [5]
RP SEQUENCE OF 293-434 FROM N.A.
RX MEDLINE; 88227861.
RA Klumpp D.J., Plank D.W., Bowdin L.J., Stueland C.S., Chung T.,
RA Laporte D.C.;
RT "Nucleotide sequence of aceK, the gene encoding isocitrate
RT dehydrogenase kinase/phosphatase.";
RL J. Bacteriol. 170:2763-2769(1988).
[Part of this file has been deleted for brevity]
FT CONFLICT 70 70 A -> R (IN REF. 2).
FT CONFLICT 80 80 A -> R (IN REF. 1 AND 2).
FT CONFLICT 116 116 I -> N (IN REF. 2).
FT CONFLICT 144 144 F -> L (IN REF. 1).
FT CONFLICT 305 312 LGEEFVNK -> WAKSSLISN (IN REF. 2).
FT CONFLICT 307 307 E -> Q (IN REF. 1).
FT STRAND 2 6
FT TURN 7 9
FT HELIX 11 23
FT TURN 26 27
FT STRAND 28 33
FT TURN 37 38
FT HELIX 39 47
FT TURN 48 48
FT STRAND 53 58
FT HELIX 64 67
FT TURN 68 69
FT STRAND 72 75
FT TURN 83 84
FT HELIX 87 108
FT TURN 110 111
FT STRAND 113 116
FT HELIX 121 134
FT TURN 135 136
FT TURN 140 141
FT STRAND 143 145
FT HELIX 148 162
FT TURN 163 163
FT HELIX 166 168
FT STRAND 173 175
FT TURN 179 181
FT STRAND 182 184
FT HELIX 186 188
FT TURN 190 191
FT HELIX 196 217
FT TURN 218 219
FT HELIX 225 242
FT TURN 243 244
FT STRAND 248 255
FT STRAND 263 271
FT TURN 272 273
FT STRAND 274 278
FT HELIX 286 311
SQ SEQUENCE 312 AA; 32337 MW; 17741A3B5AD068BA CRC64;
MKVAVLGAAG GIGQALALLL KTQLPSGSEL SLYDIAPVTP GVAVDLSHIP TAVKIKGFSG
EDATPALEGA DVVLISAGVA RKPGMDRSDL FNVNAGIVKN LVQQVAKTCP KACIGIITNP
VNTTVAIAAE VLKKAGVYDK NKLFGVTTLD IIRSNTFVAE LKGKQPGEVE VPVIGGHSGV
TILPLLSQVP GVSFTEQEVA DLTKRIQNAG TEVVEAKAGG GSATLSMGQA AARFGLSLVR
ALQGEQGVVE CAYVEGDGQY ARFFSQPLLL GKNGVEERKS IGTLSAFEQN ALEGMLDTLK
KDIALGEEFV NK
//
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> Q60150^.^1^312^SCOP^.^0^Alpha and beta proteins (a/b)^.^.^NAD(P)-binding Rossmann-fold domains^NAD(P)-binding Rossmann-fold domains^Lactate & malate dehydrogenases, N-terminal domain^KEYWORD^0.00^0.000e+00^0.000e+00 MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAVDLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLVQQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAELKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKAGGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVEERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK |
Generates DHF files from keyword search of UniProt.
Version: EMBOSS:6.4.0.0
Standard (Mandatory) qualifiers:
[-keyfile] infile This option specifies the name of keywords
file (input). This contains a list of
keywords specific to a number of SCOP or
CATH families and superfamilies used by
SEQWORDS to search a sequence database.
[-spfile] infile This option specifies the name of the
sequence database (input) to search.
[-outfile] outfile [test.hits] This option specifies the name
of the DHF file (domain hits file) (output).
A 'domain hits file' contains database hits
(sequences) with domain classification
information, in the DHF format (FASTA-like).
The hits are relatives to a SCOP or CATH
family (or other node in the structural
hierarchies) and are found from a search of
a sequence database. Files containing hits
retrieved by PSIBLAST are generated by using
SEQSEARCH, hits retrieved by a sparse
protein signatare by using SIGSCAN or
various types of HMM and profile by using
LIBSCAN.
Additional (Optional) qualifiers: (none)
Advanced (Unprompted) qualifiers: (none)
Associated qualifiers:
"-outfile" associated qualifiers
-odirectory3 string Output directory
General qualifiers:
-auto boolean Turn off prompts
-stdout boolean Write first file to standard output
-filter boolean Read first file from standard input, write
first file to standard output
-options boolean Prompt for standard and additional values
-debug boolean Write debug output to program.dbg
-verbose boolean Report some/full command line options
-help boolean Report command line options and exit. More
information on associated and general
qualifiers can be found with -help -verbose
-warning boolean Report warnings
-error boolean Report errors
-fatal boolean Report fatal errors
-die boolean Report dying program messages
-version boolean Report version number and exit
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| Qualifier | Type | Description | Allowed values | Default |
|---|---|---|---|---|
| Standard (Mandatory) qualifiers | ||||
| [-keyfile] (Parameter 1) |
infile | This option specifies the name of keywords file (input). This contains a list of keywords specific to a number of SCOP or CATH families and superfamilies used by SEQWORDS to search a sequence database. | Input file | Required |
| [-spfile] (Parameter 2) |
infile | This option specifies the name of the sequence database (input) to search. | Input file | Required |
| [-outfile] (Parameter 3) |
outfile | This option specifies the name of the DHF file (domain hits file) (output). A 'domain hits file' contains database hits (sequences) with domain classification information, in the DHF format (FASTA-like). The hits are relatives to a SCOP or CATH family (or other node in the structural hierarchies) and are found from a search of a sequence database. Files containing hits retrieved by PSIBLAST are generated by using SEQSEARCH, hits retrieved by a sparse protein signatare by using SIGSCAN or various types of HMM and profile by using LIBSCAN. | Output file | test.hits |
| Additional (Optional) qualifiers | ||||
| (none) | ||||
| Advanced (Unprompted) qualifiers | ||||
| (none) | ||||
| Associated qualifiers | ||||
| "-outfile" associated outfile qualifiers | ||||
| -odirectory3 -odirectory_outfile |
string | Output directory | Any string | |
| General qualifiers | ||||
| -auto | boolean | Turn off prompts | Boolean value Yes/No | N |
| -stdout | boolean | Write first file to standard output | Boolean value Yes/No | N |
| -filter | boolean | Read first file from standard input, write first file to standard output | Boolean value Yes/No | N |
| -options | boolean | Prompt for standard and additional values | Boolean value Yes/No | N |
| -debug | boolean | Write debug output to program.dbg | Boolean value Yes/No | N |
| -verbose | boolean | Report some/full command line options | Boolean value Yes/No | Y |
| -help | boolean | Report command line options and exit. More information on associated and general qualifiers can be found with -help -verbose | Boolean value Yes/No | N |
| -warning | boolean | Report warnings | Boolean value Yes/No | Y |
| -error | boolean | Report errors | Boolean value Yes/No | Y |
| -fatal | boolean | Report fatal errors | Boolean value Yes/No | Y |
| -die | boolean | Report dying program messages | Boolean value Yes/No | Y |
| -version | boolean | Report version number and exit | Boolean value Yes/No | N |
% seqwords Generates DHF files from keyword search of UniProt. Keywords file: seqwords.terms Swissprot-format database file: seqwords.seq Domain hits output file [test.hits]: seqwords.dhf |
Go to the input files for this example
Go to the output files for this example
| FILE TYPE | FORMAT | DESCRIPTION | CREATED BY | SEE ALSO |
| Domain hits file | DHF format (FASTA-like). | Database hits (sequences) with domain classification information. The hits are relatives to a SCOP or CATH family (or other node in the structural hierarchies) and are found from a search of a discriminating element (e.g. a protein signature, hidden Markov model, simple frequency matrix, Gribskov profile or Hennikoff profile) against a sequence database. | SEQSEARCH (hits retrieved by PSIBLAST). SIGSCAN (hits retrieved by sparse protein signature). LIBSCAN (hits retrieved by various types of HMM and profile). | N.A. |
| Keywords file | Text | Contains a list of keywords specific to a number of SCOP families and superfamilies used by SEQWORDS to search a sequence database. | N.A. | N.A. |
| Program name | Description |
|---|---|
| cathparse | Generates DCF file from raw CATH files |
| domainalign | Generate alignments (DAF file) for nodes in a DCF file |
| domainnr | Removes redundant domains from a DCF file |
| domainrep | Reorder DCF file to identify representative structures |
| domainseqs | Adds sequence records to a DCF file |
| domainsse | Add secondary structure records to a DCF file |
| helixturnhelix | Identify nucleic acid-binding motifs in protein sequences |
| libgen | Generate discriminating elements from alignments |
| matgen3d | Generate a 3D-1D scoring matrix from CCF files |
| pepcoil | Predicts coiled coil regions in protein sequences |
| rocon | Generates a hits file from comparing two DHF files |
| rocplot | Performs ROC analysis on hits files |
| scopparse | Generate DCF file from raw SCOP files |
| seqalign | Extend alignments (DAF file) with sequences (DHF file) |
| seqfraggle | Removes fragment sequences from DHF files |
| seqsort | Remove ambiguous classified sequences from DHF files |
| ssematch | Search a DCF file for secondary structure matches |
See also http://emboss.sourceforge.net/